Job Information
Rockefeller University Research Assistant | Laboratory of Cell Cycle Genetics in New York, New York
Organization Overview
Cell cycle control involves coordinated production and destruction of proteins that activate cyclical events required for precise cell duplication. Headed by Dr. Frederick R. Cross, the Laboratory of Cell Cycle Genetics uses a variety of approaches to investigate cell cycle control at the molecular level.
Overview
The overall purpose of the project is to search for broadly cross-reactive single-chain antibodies against viruses in the coronavirus family. Specifically the work will consist of assisting in: construction of libraries of yeast expressing single-chain antibodies against multiple viral antigens in the coronavirus family; screening the libraries for binding and cross-binding to antigens from related viruses; DNA preparation and analysis of next-generation sequencing data to determine sequences and specificities of cross-binding vs. specific antibodies.
Responsibilities
Responsibilities include:
Prepare plasmid and PCR-generated DNA for construction of yeast libraries
Carry out high-efficiency yeast transformation to generate complex libraries
Select differential binding clones using magnetic bead technology
Purify DNA from selected populations, prepare for next-generation sequencing
Perform related duties and responsibilities as assigned/requested
Qualifications
Bachelor of Science degree with basic laboratory experience is required. Specific experience in molecular biology and microbiology (DNA preparation and analysis, media preparation, microbial culture) is strongly preferred.
Rockefeller University does not discriminate in employment on the basis of race, color, religion, sex (including pregnancy, gender identity), national origin, political affiliation, sexual orientation, marital status, disability, genetic information, age, membership in an employee organization, retaliation, parental status, military service or other non-merit factor. All qualified applicants will receive consideration for employment without regard to the characteristics listed above.
Compensation Range: Min
USD $46,000.00/Yr.
Compensation Range: Max
USD $50,000.00/Yr.
Job Locations US-NY-New York
Category Science
Position Type Regular Full-Time