Rockefeller University Research Specialist, Drug Discovery in New York, New York
Job Title Research Specialist, Drug Discovery
Laboratory / Department High Throughput Screening Resource Center
The High-Throughput and Spectroscopy Resource Center (HTSRC) supports scientists in the screening and identification of compounds and genetic modulators of in vitro assays which utilize optical and other bio-analytical technologies. High-Throughput Screening (HTS) requires the miniaturization and automation of bioassays so that millions of variables can be tested. HTS is often an important step in the discovery of new medicines. The center has a collection of over 380,000 compounds, automated liquid transfer devices, compound databases, and supports a broad diversity of assay development techniques, typically found in early drug discovery programs. The Center is configured for processing 96, 384 and 1536-well microtiter plates through the use of automated changeable tip dispensers, non-contact dispensers, and syringe dispensers. For assay technologies, the HTSRC has the capability to support cellular and biochemical assays using absorbance, fluorescent kinetics, fluorescence anisotropy, time-resolved fluorescence, time-resolved fluorescence resonance energy transfer, AlphaScreen, bioluminescence, for example luciferase and green fluorescent protein, scintillation proximity and cellular fluorescence imaging. Assay targets can include ion channels, receptors, enzymes, protein interactions, signaling pathways and cellular processes.
The High-Throughput and Spectroscopy Resource Center also oversees the management of, training for, and scheduling for optical spectroscopic and calorimetric equipment for use in studies of the structure, function and interactions of biological and organic molecules. This includes an on-campus facility for nuclear magnetic resonance spectroscopy with priority access given to University researchers. The Center offers extensive training in basic and advanced NMR experiments and also coordinates access to high field spectrometers (700, 800, and 900 MHz) at the New York Structural Biology Center through the University's partnership in that Center. HTSRC staff provides general background training in applications of NMR spectroscopy to structural biology and chemistry and hands on training on the spectrometers. Trained users have 24/7 access to these instruments. Occasional users of the facilities may have spectra produced for them.
The Research Support Specialist will be responsible for developing and conducting biochemical assays, cell-based and biophysical assays, and high throughput screens. Will train technicians, graduate students, and post-docs in the technical implementation of tool and drug discovery projects, design of HTS experiments, and use of instruments. The Research Support Specialist will evaluate new technologies and approaches for HTS and spectroscopy, as well as contribute to the writing of scientific manuscripts and composition of methods, figures and figure legends for published materials. Responsibilities will also include maintaining and independently troubleshooting equipment, including microplate readers, high-throughput microscopes, spectroscopic instruments, and automated liquid dispensers
Ph.D. in biology, chemistry, or a related field required. Must have previous experience operating, troubleshooting, and maintaining quality control for automated pipetting workstations, automated storage systems, and microplate readers. Must have previous experience formatting compound plates for single point concentrations and concentration-response experiments, as well as developing high throughput screening assays involving a wide variety of fluorescent and cell-based modalities. Must be skilled in data organization, analysis, entry and interpretation.
The Rockefeller University is an Equal Opportunity Employer - Minorities/Women/Disabled/Veterans